Establishment of efficient regeneration protocol for three rapeseed cultivars

Abstract

Economically, rapeseed is one of the most important crops in the world. Over the past decades, rapeseed research has been focused on improving biotechnological methods to facilitate breeding. The effectiveness of these methods depends on efficient tissue culture techniques. The aim of the present study was to establish an efficient protocol for regeneration of rapeseed. It was conducted in three stages. The first stage was to determine the effect of different concentrations (10%, 20%, 30% and 40%) of sodium hypochlorite solutions on seedling growth parameters. In the second stage, the effects of different growth media (Murashige and Skoog, MS, and Gamborg) and plant growth regulators (6-benzylaminopurine, 1-naphthaleneacetic acid and thidiazuron) at different concentrations on the regeneration capacity of stem explants of three rapeseed cultivars were investigated. In the last stage, the shoots of ‘Spok’ were cultured for three weeks on MS medium containing 1.5, 3 and 4.5 mg L−1 of indol butyric acid for rooting. The best results in germination, seedling growth and root length were obtained by using 10% disinfectant concentration for 25 minutes. The highest values for shoot regeneration were obtained from the stem explant cultured on MS medium containing 3 mg L−1 BAP and 0.2 mg L−1 NAA. It was found that MS containing 1.5 mg L−1 IBA was the most efficient medium for root formation.