Establishment of DNA Fingerprint Identity for Sugarcane Cultivars in Yunnan, China

Abstract

The purpose of this study was to provide simple sequence repeat (SSR)-based DNA fingerprinting information for sugarcane ( Saccharum spp. Hybrids) cultivars that were developed in Yunan Province, China. Genomic DNAs from 27 cultivars were amplified with 8 pairs of polymorphic SSR primers. A total of 129 bands were obtained, including 123 polymorphic bands (95.35%). The mean value of polymorphic information content (PIC) was 0.9445, and the genetic similarity coefficient ranged from 0.269 to 0.767. Markers SMC1047HA and MSSCIR21 had the highest PIC values of 0.963 and could distinguish all the cultivars tested. Besides, primer combinations MSSCIR36/MSSCIR21, MSSCIR16/MSSCIR36, and MSSCIR36/SMC336BS were efficient in identifying the 27 cultivars, and their similar coefficients were lower than those of other primer combinations. These 3 primer combinations were validated with 27 cultivars and 10 staple commercial sugarcane cultivars in China, and MSSCIR16/MSSCIR36 was found to be optimal to construct DNA fingerprint identity (ID). Nomenclature of DNA fingerprint ID was suggested as serial number of National Nursery of Sugarcane Germplasm Resources (NNSGR) plus country and region code plus English initial abbreviation of breeding institution plus core primer name plus SSR marker data. This system is informative and useful in protecting new varieties and identifying cultivars because it consists of not only important information of a cultivar but also its specific molecular identify.