Abstract
Objective To develop a digital polymerase chain reaction (PCR)-ribotyping method and database for Clostridium difficile genotyping. Methods Sequencer based fluorescence capillary gel electrophoresis was used, instead of agarose gel electrophoresis, to establish the digital PCR-ribotyping of Clostridium difficile. Forty Clostridium difficile reference strains, consisting of 10 PCR-ribotypes (RT), were genotyped by the new digital PCR-ribotyping method to set-up the database. Results The sequencer based fluorescence capillary gel electrophoresis correctly detected PCR-ribotyping products of the 40 reference strains, and showed as digital figure; significant differences of these digital figures were found between the 10 RT. High similar digital figures were shown in twenty-one RT027 strains, three RT002 strains and two RT014 strains. However, seven RT001 strains were typed as four subtypes, and two RT014 strains as two subtypes, respectively. Conclusion A digital PCR-ribotyping, and a reference database consisting of 10 RT are successfully established. Key words: Clostridium difficile; PCR-ribotyping; Fluorescence capillary gel electrophoresis; Digital; Database
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
