Abstract
The effects of moisture, NO 3 −1 concentration and C addition on changes in denitrification capacity and total microbial biomass in a clay loam soil were investigated. Denitrification capacity was evaluated with an anaerobic slurry technique. Total microbial biomass was measured by CHC1 3 fumigation and by extraction of microbial ATP. The results indicate that denitrification capacity and total microbial biomass were increased only by the C addition; differences in NO 3 −1 concentration and moisture had no effect in this agricultural soil. The increase in denitrification capacity could be attributed solely to microbial growth, since the ratio of denitrification capacity to total microbial biomass remained constant and the increased respiration from the C amendment did not increase anaerobiosis. The results also show that denitrifiers compete as effectively for added C as do other heterotrophs.
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