Establishment of coronary heart disease models of Qi-deficiency and blood-stasis syndrome in rats

Abstract

To establish Qi-deficiency and blood-stasis syndrome type coronary heart disease models by fatigue running exercise and high ligation of left anterior descending coronary artery (LAD), male Wistar rats were selected and randomly divided into sham operation group (JSS), coronary ligation group (DZ), fatigue running exercise+coronary ligation group (PZ). Coronary ligation alone was done in DZ group; while the rats in PZ group had running exercise in on the animal treadmill system for 2 weeks to establish fatigue models, and then coronary ligation was done based on the models. The exhausted running was maintained for 28 days at the frequency of 1 time/2 days after operation. Twenty-eight to thirty-one days after the operation, all the rats were observed for macroscopic physical signs, and ultrasonic echocardiography indexes and breathing extent of the rats were collected to evaluate the main symptoms of rats with Qi-deficiency and blood-stasis syndrome type coronary heart disease; related indexes of open field test, exhaustive running time, and colorimetric analysis data on images of plantar were collected to evaluate the accompanied symptoms; colorimetric analysis data on lingual surface was collected to evaluate the tongue characteristics; pulse distension data was collected to evaluate the pulse condition, and meanwhile, blood rheology and coagulation function were also detected. From the 28th day postoperatively, the main symptoms, accompanied symptoms, tongue characteristics and pulse conditions of rats in PZ group conformed to the symptoms of coronary heart disease and Qi-deficiency and blood-stasis syndrome. Combined with related pathological results, the study revealed that Qi-deficiency and blood-stasis syndrome type coronary heart disease models could be successfully established by fatigue running exercise and high ligation of left anterior descending coronary artery for the rats.