Establishment of colloidal gold immunochromatography strip for rapid detection of Karen mikimotoi and its application.

Abstract

In China, harmful algal blooms (HABs) are one of the most prominent ecological disasters in the coastal areas. Among the harmful algae species that cause HABs, Karen mikimotoi is a kind of algae that appear frequently. It can secrete hemolytic toxins and fish toxins such as glycolipids and unsaturated fatty, posing a significant threat to marine life. In order to establish a fast and effective detection technology for Karen mikimotoi that can be promoted and applied on site, we have developed a test strip which is based on monoclonal antibody technology and the colloidal gold immune-chromatography assay (GICA). The experimental results show that this test strip can detect different growth stages including growth, and stable and recession period of Karen mikimotoi. The detection results can be displayed within 3-15min. It had high sensitivity and specificity, with a detection limit of 754 cells/mL. A colorimetric card was made to further determine the concentration of algae detected. What is more, we had developed a method that can be used for on-site enrichment of algae cells using a syringe to detect lower concentrations of Karen mikimotoi, with a minimum detection concentration of 100 cells/mL. Also the test strip was used for on-site testing along the coast of China. This test strip not only serves as a warning for the outbreak of red tide, but also provides a new approach for the development of rapid detection technology for red tide algae.