Abstract
The specificity of circulating tumor cell (CTC) such as epithelial-mesenchymal transition,fusion of bone marrow-derived cells,resistance anoikis determines the necessity of cell cultivating.The produced cell lines can provide good material basis for further research of malignant tumor metastasis,also provide individualized targeted therapy for patients with a new direction. Key words: Neoplasm circulating cells; Cell culture techniques; Cell line, tumor
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