Abstract

1) Two cell lines were established in vitro from a mouse ascites mammary carcinoma FM3A and they were similar to each other both in their in vitro growth rate and their morphological identity with in vivo tumor cells. 2) Both A and B lines had three characteristic chromosomes and high transplantability, and the transplanted subcutaneous tumors showed the morphology of undifferentiated adenocarcinoma. 3) The cloning of the present cell lines were made with the use of agar. 4) The study on the nutritional requirement of the present lines showed that Bactopepton, pyruvate and serine were growth enhancing respectively and the combination of these additives were more effective than single addition. 5) The glucose utilization of the lines in the course of the growth was enhanced at the growth stage of the lag phase and early logarithmic phase but decreased after the stage of late logarithmic phase. 6) The capacity to grow in vitro without lag time could be maintained for a pretty long time after transplantion to the host animal, but it fell gradually.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.