Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production.

Abstract

In the present study, a protocol was optimized for establishment of callus and cell suspension culture of Scrophularia striata Boiss. as a strategy to obtain an in vitro acteoside producing cell line for the first time. The effects of growth regulators were analyzed to optimize the biomass growth and acteoside production. The stem explant of S. striata was optimum for callus induction. Modified Murashige and Skoog medium supplemented with 0.5mg/l naphthalene acetic acid+2.0mg/l benzyl adenine was the most favorable medium for callus formation with the highest induction rate (100%), the best callus growth and the highest acteoside content (1.6μg/g fresh weight). Incompact and rapid growing suspension cells were established in the liquid medium supplemented with 0.5mg/l naphthalene acetic acid+2.0mg/l benzyl adenine. The optimum time of subculture was found to 17-20days. Acteoside content in the cell suspension was high during exponential growth phase and decreased subsequently at the stationary phase. The maximum content of acteoside (about 14.25μg/g cell fresh weight) was observed on the 17th day of the cultivation cycle. This study provided an efficient way to further regulation of phenylethanoid glycoside biosynthesis and production of valuable acteoside, a phenylethanoid glycoside, on scale-up in S. striata cell suspension culture.