Abstract
A blood-brain barrier(BBB) model for the screening of drug candidates was established by means of ECV304/C6 co-culture.The permeability parameters and morphological analysis showed that the cultured ECV304 cell monolayer was of integrity and vitality.The active components of widely used medicinal plant Danshen(Salvia miltiorrhiza) were screened with BBB model,and then analyzed and elucidated with LC-MS.The results indicated that 16 compounds at least,in Danshen extracts,could permeate over BBB model,in which cryptotanshinone,tanshinone I,danshensu and protocatechuic acid were deduced and identified.Quantitative structure activity relationship analysis further showed that the identified 4 compounds were consistent with the rules for central nervous system drug-like molecules.This result demonstrated that the combination of ECV304/C6 co-culture model with LC-MS analysis should be an attractive and great potential method for the screening of central nervous system drug candidates from traditional Chinese medicine.
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