Establishment of animal model of in situ transplanted tumor of human pancreatic cancer MIA PaCa II cells with activity of nervous invasion

Abstract

Objective To establish the nude mice model of in situ transplanted tumor of human pancreatic cancer MIA PaCa Ⅱ cells and observe its activity of nervous invasion to provide experimen-tal basis and condition for study the occurrence and development of the nervous invasion by pancreatic cancer. Methods The human pancreatic cancer MIA PaCa Ⅱ cells were implanted on back of nude mice to establish the subcutaneous model of transplanted tumor of pancreatic cancer with high activity of metastasis in nude mice. Then the tumor was implanted in situ in the pancreas of the nude mice.The nude mice was sacrificed in 4, 6 and 8 weeks for anatomy and pathological examination of the transplanted tumor as well as determination of its size and weight. Furthermore, HE staining and sil-ver staining were employed to observe its activity of nervous invasion. Results During the establish-ment of subcutaneous model, the successful rate of implantation was 100% (10/10). The subcutane-ous transplanted tumor had limited growth and there was no metastasis to organs and nerves. In 4, 6 and 8 weeks after the in situ pancreatic implantation, the successful implantation rate was 100% (10/ 10). The rate of nervous invasion was 50% (5/10), 80% (8/10) and 60% (6/10), respectively.Meanwhile, there were metastases of the tumor in various organs. Conclusion The in situ transplan-ted tumor model was successfully established in nude mice and its nervous invasion observed. This model is a fairly optimal model for study of nervous invasion by pancreatic cancer. Key words: Pancreatic neoplasms; Model; Nerve; Metastasis