Abstract
Dermal papilla (DP) plays important roles in hair follicle regeneration. Long-term culture of mouse DP cells can provide enough cells for research and application of DP cells. We optimized the culture strategy for DP cells from three dimensions: stepwise dissection, collagen I coating, and optimized culture medium. Based on the optimized culture strategy, we immortalized primary DP cells with SV40 large T antigen, and established several immortalized DP cell strains. By comparing molecular expression and morphologic characteristics with primary DP cells, we found one cell strain named iDP6 was similar with primary DP cells. Further identifications illustrate that iDP6 expresses FGF7 and α-SMA, and has activity of alkaline phosphatase. During the process of characterization of immortalized DP cell strains, we also found that cells in DP were heterogeneous. We successfully optimized culture strategy for DP cells, and established an immortalized DP cell strain suitable for research and application of DP cells.
Highlights
Hair follicles have the characteristic of periodical growth, which provides a nice model for the research of tissue regeneration
We found that a mesenchymal stem cell culture medium α-MEM worked well
From the results of present study, it can be concluded that we optimized the dissection and culture of mouse Dermal papilla (DP) cells from three dimensions: stepwised dissection, collagen I coated plate and α-MEM based culture medium
Summary
Hair follicles have the characteristic of periodical growth, which provides a nice model for the research of tissue regeneration. The signals from DP may regulate the regeneration of hair follicles and melanocyte (Guo et al, 2016; Li et al, 2013). Dissociated human DP cells induce hair follicle neogenesis in grafted dermal-epidermal composites (Thangapazham et al, 2014). The limitation for DP research lies in the difficulty for culture of DP cells (Morgan, 2014). The human intact dermal papilla transcriptional signature can be partially restored by growth of papilla cells in 3D spheroid cultures (Topouzi et al, 2017). When the culture environment was changed into 2D environment, very rapid and profound molecular signature changes were discovered (Higgins et al, 2013; Lin et al, 2016). The isolation method of DP by surgical microdissection has been established in mouse vibrissae
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
