Establishment of an effective oligotrophic cultivation system for Rhodococcus erythropolis N9T-4.

Tomohiro Matsuoka,Nobuyuki Yoshida

doi:10.1080/09168451.2018.1482196

Tomohiro Matsuoka, Nobuyuki Yoshida

Open Access

https://doi.org/10.1080/09168451.2018.1482196

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Abstract

Rhodococcus erythropolis N9T-4 grows on an inorganic solid-state medium with no additional carbon and energy sources; however, it is unable to grow well in a liquid culture medium under the oligotrophic conditions. We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium.

Highlights

To evaluate the N9T-4 cells grown with the sponge, the activities of the key enzymes involved in the oligotrophic growth of N9T-4 were measured
We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium
It has been elucidated that N9T-4 can utilize trace amounts of ammonia in the atmosphere as the nitrogen sources, and we suggest that this bacterium can utilize aldehyde and sulfur compounds in the atmosphere [5,7]

Summary

Introduction

To evaluate the N9T-4 cells grown with the sponge, the activities of the key enzymes involved in the oligotrophic growth of N9T-4 were measured. Rhodococcus erythropolis N9T-4 grows on an inorganic solid-state medium with no additional carbon and energy sources; it is unable to grow well in a liquid culture medium under the oligotrophic conditions. We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium.

Results

Conclusion