Abstract
Rhodococcus erythropolis N9T-4 grows on an inorganic solid-state medium with no additional carbon and energy sources; however, it is unable to grow well in a liquid culture medium under the oligotrophic conditions. We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium.
Highlights
To evaluate the N9T-4 cells grown with the sponge, the activities of the key enzymes involved in the oligotrophic growth of N9T-4 were measured
We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium
It has been elucidated that N9T-4 can utilize trace amounts of ammonia in the atmosphere as the nitrogen sources, and we suggest that this bacterium can utilize aldehyde and sulfur compounds in the atmosphere [5,7]
Summary
To evaluate the N9T-4 cells grown with the sponge, the activities of the key enzymes involved in the oligotrophic growth of N9T-4 were measured. Rhodococcus erythropolis N9T-4 grows on an inorganic solid-state medium with no additional carbon and energy sources; it is unable to grow well in a liquid culture medium under the oligotrophic conditions. We examined submerged cultivations of N9T-4 using a polyurethane foam sponge to achieve approximately 10 times of the oligotrophic growth of the bacterium in the liquid culture medium.
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