Abstract
Biological control of grey mould caused byBotrytis cinerea is achieved byTrichoderma harzianum under commercial greenhouse conditions. The establishment of populations ofTrichoderma in the phylloplane of treated plants was studied. After several applications of a formulated preparation, a level of 105 cfu per leaf was established. High relative humidity (r.h.) but not saturation favoured establishment and biocontrol activity ofT. harzianum.T. harzianum affected the level of natural bacterial and fungal populations, resulting in an increase or decrease in comparison with nontreated leaves. However, r.h., temperature, plant nutrition, plant species, and leaf age influence the effect of the biocontrol agent on the indigenous microflora. It is suggested that microclimatic conditions affectTrichoderma establishment more than does plant nutrition.
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