Establishment of a split liver transplantation pig model using extracorporeal normothermic machine perfusion

Abstract

Objective To summarize the experience of establishing a split liver transplantation pig model using extracorporeal normothermic machine perfusion (NMP). Methods Twenty miniature pigs were purchased with ten as donors and another ten as receptors. The graft was spliced along Taira line and the right half was reserved for transplantation. Hemodynamics and bile production volume were monitored, and blood biochemical and blood gas analysis indicators were detected during machine perfusion. Pathological change was observed by HE stain. Hemodynamics during liver transplantation, 5-day survival rate and the cause of death were recorded. Results Hemodynamic, biochemical and blood gas analysis indicators remained stable during NMP. All receptor pigs were successfully extubated and awake after surgery. Two receptors died on the second day after the operation. The 5-day survival rate was 80%. Conclusion The split liver transplantation pig model using extracorporeal normothermic machine perfusion is feasible and appropriate, and it lays the foundation for further investigation. Key words: Split liver transplantation; Pig; Normothermic machine perfusion