Abstract
Chlorogenic acid [3-(3,4-dihydroxycinnamoyl)quinic acid, CGA] is one of the main compounds contained in immature pear fruits. In the present study, we established simple purification of CGA in high purity from the immature fruit of pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) which contains CGA in high concentration. The 80 % MeOH extract (1.4 g) of immature pear fruits (20 g fresh wt.) was partitioned with ethyl acetate (EtOAc) under acidic condition (pH 2.65). Then, the EtOAc-soluble acidic layer (110.3 mg) was further purified using Diaion HP-20 column chromatography (H2O/MeOH, step wise). Then, the CGA-contained fractions were finally purified by preparative ODS-HPLC. The isolated CGA (24.3 mg) showed high purity (>99 %) and the total recovery was estimated to be 63.9 %.
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