Abstract
Ground-cover Chrysanthemum has been used in gardens due to its rich colors, excellent ornamental value. Too much precipitation in summer of north China limits its further use. In order to create chrysanthemum with high resistance to waterlogging, the Vgb (Vitreoscilla hemoglobin) and pmi (phosphomannose isomerase) genes are transferred into the genome of ground-cover chrysanthemum ‘Jindinghongxin’ by Agrobacterium tumefaciens mediated transformation in our research. This research is of profound importance to broaden the usage of ground-cover chrysanthemum in gardens. This research optimized the regeneration system of ‘Jindinghongxin’ including choosing young leaves as explants and the best regeneration culture medium (MS+2.0 mg/L 6-BA+0.5 mg/L NAA), Transformation system for ‘Jindinghongxin’ was established by pre-culturing leaf explants for 2 days. The suitable concentration of Agrobacterium tumefaciens and the suitable dipping time for transformation was OD600=0.4 for 5 minutes. Co-cultivation was done for 2 days at 28°C in the dark after infection and the explants were then put in delayed culture for 4 days. Then the explants were cultured in first selection medium with 6 g/L mannose for 15 days, and then cultured in second selection medium with 7 g/L mannose. Once the regeneration shoot was 1 cm long it was transformed into MS with 350 mg/L carbenicillin for root induction.
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