Establishment of a Rapid Plant Regeneration System in &lt;i&gt;Physalis angulata&lt;/i&gt; L. through Axillary Meristems

Owk Aniel Kumar,Sape Subba Tata,Songa Ramesh

doi:10.15835/nsb749707

Abstract

An optimal plant propagation method of Physalis angulata L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum in vitro response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA3) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective in vitro shoot elongation and rooting was achieved on 1.0mg/L(GA3) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent. This protocol can be used for commercial propagation and for future genetic improvement studies.

Highlights

Medicinal plants play a crucial role in health care needs of people around the world especially in developing countries (Rao et al, 2004; Bekalo et al, 2009)
The present study describes the maximization of shoot multiplication through in vitro regeneration of Physalis angulata L. by axillary meristem explants using standard culture medium fortified with different plant growth regulators
Axillary meristem explants were excised from natural plants and were cultured on different plant growth regulator (PGR) regimes

Summary

Introduction

Medicinal plants play a crucial role in health care needs of people around the world especially in developing countries (Rao et al, 2004; Bekalo et al, 2009). Plant shows good antibacterial and antifungal activities (Osho et al, 2010) Natural population of this plant species is decreasing due to indiscriminate exploitation and this has been the trend for decades in developing countries (Navdeep et al, 2015). The plant regeneration from meristems is considered to be one of the most promising ways for multiplying a selected variety true to its type Such individuals are genetically similar showing the same agronomic characteristics. The present study describes the maximization of shoot multiplication through in vitro regeneration of Physalis angulata L. by axillary meristem explants using standard culture medium fortified with different plant growth regulators. The protocols presented here should become a valuable part of future efforts in genetic improvement of Physalis angulata

Materials and Methods

Results and Discussion

Conclusions