Establishment of a rabbit model of spinal tuberculosis with mycobacterium tuberculosis H37Rv

Abstract

Objective Drill a hole in the lumbar vertebral of the rabbit and implant human type H37 Rv tuberculosis standard strain in the hole to construct the model of spinal tuberculosis of the rabbit.Methods Drill holes at the top of the 5th anodic lumbar vertebrae in 16 rabbits,and fill the holes with gelatin sponge to adsorb the M.tuberculosis suspension 0.1 ml ( the amount of bacteria:5 g/L).Finally,close the holes with bone wax.After operation,adopt examinations of general observation,Imaging,Histopathology,Bacteriology etc at different time periods.Results 13 rabbits whose lumbar vertebral bodies were infected had obvious partial response and lesser response of whole bodies.3 of the 16 rabbits were not infected by mycobacterium tuberculosis.2 of the infected ones appeared to have weight loss,anorexia and other symptoms.One month after operation,4 rabbits' X-ray findings were positive; 4 months after operation,8 rabbits' X-ray findings were positive,12 rabbits' CT findings were realized as positive and 13 rabbits' MRI findings were realized as positive; From the vertebral body and paravertebral soft tissue sections of the infected rabbits we can see the formation of pus cells,epithelioid cells or necrosis.Purulent Juice Culture on abscesses shows that 69.2％ of mycobacterium tuberculosis grew.The success rate of Modeling is 69.2％.Conclusion By implanting proper dosage of human type H37Rv tuberculosis standard strain in the local lumbar vertebral of the rabbit,we can build spinal tuberculosis model of rabbit which is similar to the human spinal TB management changes. Key words: Spinal tuberculosis; Mycobacterium tuberculosis; Model, animal