Establishment of a Particle Concentration Fluorescence Immunoassay (PCFIA) for the Measurement of Human IgG in Culture Supernatants

Abstract

It is well established that antibodies of the IgG isotype are important for resisting infections. The measurement of IgG levels in supernatants of pokeweed mitogen (PWM) stimulated peripheral blood lymphocytes (PBL) is widely used in clinical laboratories to assess B and T cell function, and similar cultures are routinely run in many laboratories to study the T cell subsets involved in the regulation of IgG synthesis by B cells. Although ELISA and RIA assays are widely used for the detection of IgG in culture supernatants, a sensitive, fast and automated assay would be advantageous.