Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars

Abstract

Leguminous Broad bean (Vicia faba L.) is an excellent source of minerals, vitamins and proteins that makes it higly valuable food for human and feed for animal consumption. The plant has an important characteristic to fix atmospheric Nitrogen and play an important role to establish a natural balance of N in the atmosphere. It is a highly self-pollinated plant and has problem of low variation with limited genetic pool. Moreover, the previous studies identify problem of recalcitrance in broad bean. Therefore, there is need to establish a repeatable micropropagation protocol that could ensure an increase in genetic variability to overwhelm problems in breeding. This system must also be able for efficient gene delivery and could be integrated with the conventional breeding programs through direct organogenesis. The study aimed to develop a tissue culture protocol on two important Turkish broad bean cultivars Filiz99 and Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mg l-1 TDZ using embryonic axis as explant. Maximum number of 5.33 and 3 shoots per explant were noted on cv. Filiz99 and Eresen87 on MS medium containing 0.15 mg l-1 TDZ. The developing shoots were rooted on MS medium containing 1 mg l-1 IAA after three weeks of culture. The rooted plants were transferred to pots containing peat under maintained under controlled greenhouse conditions for acclimatization The acclimatized plants bloomed and set seeds. Present results underscore importance of seed hydropriming before taking of explants to achieve high micropropagation on faba beans to overcome recalcitrance.