Abstract
Objective To build a convenient model of pancreas ischemia reperfusion injury (IRI)in rats for investigating its negative impact on pancreas. Methods Wistar rats were randomized into 4groups:(1)sham-operated animals with dissociation of the pancreatic tail-segment ( sham, n=20 ); ( 2 )control animals with dissociation of the pancrea-tic tail-segment, 30 seconds of ischemia by bcloking the abdominal aorta and flushing(control, n=20);(3) Group New model animals experienced with dissociation of the pancreatic tail-segment, flushing, 120 mins of ischemia and reperfusion (IR, n=20). The level of serum amylase and lipase, wet to weight ratio of pancreas and pathological changes of pancreas were observed at0, 2, 4, 6 hours after reperfusion. Results The level of serum amylase[2 h (3127.80±150.85) U/L, 4 h (3122.80±131.52) U/L, 6 h (2585.20±161.06) U/L]and lipase[2 h (446.00±181.54) U/L, 4 h (517.40±165.22) U/L, 6 h (475.20 ± 170.37) U/L]in Group New model were increased significantly after perfusion accordingly, The tissue damage of pancreas become more serious after perfusion. Conclusion The model shows a typical IRl on pancreas of rats. It's a ideal and clinically relevant animal model for the study of pancreas IRI after transplantation. Key words: Reperfusion injury; Pancreas; Model, animal
Published Version
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