Abstract
Background: Sweat is a major aggravating factor of atopic dermatitis (AD) and approximately 80% of patients with AD reveal type I-hypersensitivity against sweat and semi-purified sweat antigen (QRX). To diagnose the sweat allergy, we routinely perform the basophil histamine release test (HRT). However, HRT needs fresh blood cells of patients and its results are qualitative rather than quantitative. In this study, to overcome the disadvantages of HRT, we established the enzyme-linked immunosorbent assay (ELISA) to measure specific IgE against sweat antigen by using sera of patients with AD.
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