Abstract
Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 mast cells could be maintained without additional growth factors except fetal calf serum. NCL-2 mast cells expressed FcepsilonRI and KIT on their surface, and release histamine and LTB4 in response to antigen stimulation. In contrast to P815 mast cells, which express a constitutively- active KIT, NCL-2 cells shows slower, non-lethal tumor growth when inoculated in the skin of Balb/c nude mice. NCL-2 cells shows tyrosine phosphorylation of KIT and Jak2 in response to SCF and IL-3 stimulation, respectively. Moreover, the expression pattern of mast cell proteases in NCL-2 mast cells changed by the stimulation of SCF and IL-3. In conclusion, NCL-2 mast cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
