Abstract
Human umbilical vein endothelial cells (HUVECs) were infected with a recombinant amphotropic retrovirus containing the E6 and E7 genes of human papillomavirus type-16 (HPV-16) and the neomycin resistance gene. The cells were subsequently selected in G418 and 10 clones of resistant cells isolated and expanded for analysis. All 10 clones were of endothelial origin as determined by positive staining for Factor VIII related antigen. Functional data suggested that the clones retained the properties of normal HUVECs. All clones entered a period of crisis after about 50 generations characterised by the presence of giant, multinucleate dendritic cells. One clone emerged from this crisis period and has been established as a cell line named HPVEC4. HPVEC4 has been fully characterised indicating that it still retains all of the endothelial characteristics exhibited before entry into crisis. Specifically, HPVEC4 is able to (a) differentiate into capillaries when plated onto gels composed of basement membrane componen...
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