Abstract
Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.
Highlights
With approximately one million newly diagnosed cases annually, gastric cancer poses a serious threat to the health and lives of people worldwide [1, 2]
Human-derived gastric cancer xenograft models have been established in immunodeficient mice, such as nude mice and mice with severe combined immunodeficiency
The establishment of a novel human gastric cancer xenograft model in mice with a normal immune system is of great significance [5, 6]
Summary
With approximately one million newly diagnosed cases annually, gastric cancer poses a serious threat to the health and lives of people worldwide [1, 2]. The pathogenesis and mechanisms of metastasis of gastric cancer have yet to be completely clarified. In vivo models of gastric cancer are essential tools for exploration of the biological characteristics of this cancer and potential novel treatment options [3, 4]. Human-derived gastric cancer xenograft models have been established in immunodeficient mice, such as nude mice and mice with severe combined immunodeficiency. Immunodeficient mice do not reflect the important roles of the immune system in tumor development and progression. The establishment of a novel human gastric cancer xenograft model in mice with a normal immune system is of great significance [5, 6]. MKN45, human gastric cancer cells and the microcarrier were cocultured, and immunocompetent mice were subsequently
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
