Abstract
A human clonal cell line, designated TAH9, has been established from cells of adipose tissue by fusion with E. coli protoplasts containing a recombinant plasmid carrying the early genes of Simian virus 40. The establishment of the cell line is preceeded by a delayed growth crisis. The results indicate that, after cell transformation, another event(s) of low frequency is required for cell immortalization. Some biological properties of TAH9 cells are also described.
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