Abstract
A host-vector system was constructed in Bacillus megaterium strain NK84–0128, an oxetanocin A producer. The replication origin of an endogeneous plasmid, P–4, was used to construct a potential plasmid vector, pSM5, which had a chloramphenicol resistance gene as a selective marker. Plasmid transformation by a protoplast method was used in B. megaterium strain NK84–0128. The maximum transformation frequency attained with the pSM5 plasmid was 2.0 x 104cfu/µg DNA.
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