Abstract
The objective of this study was to develop a bovine viral diarrhea virus type 2 (BVDV-2) challenge model suitable for evaluation of efficacy of BVDV vaccines; a model that mimics natural infection and induces clear leukopenia and viremia. Clinical, hematological and virological parameters were evaluated after infection of two age groups of calves (3 and 9 months) with two BVDV-2 strains (1362727 and 502643). Calves became pyrexic between 8 and 9 days post inoculation and exhibited symptoms, such as nasal discharge, mild depression, cough, and inappetence. Leukopenia with associated lymphopenia and neutropenia was evident in all groups with lowest leukocyte and lymphocyte counts reached 8 dpi and granulocyte counts between 11 and 16 dpi, dependent on the strain and age of the calves. A more severe thrombocytopenia was seen in those animals inoculated with strain 1362727. Leukocyte and nasal swab samples were positive by virus isolation, as early as 3 dpi and 2 dpi respectively, independent of the inocula used. All calves seroconverted with high levels of BVDV-2 neutralizing antibodies. BVDV RNA was evident as late as 90 dpi and provides the first evidence of the presence of replicating virus long after recovery from BVDV-2 experimental infection. In summary, moderate disease can be induced after experimental infection of calves with a low titer of virulent BVDV-2, with leukopenia, thrombocytopenia, viremia, and virus shedding. These strains represent an attractive model to assess the protective efficacy of existing and new vaccines against BVDV-2.
Highlights
Bovine viral diarrhea virus (BVDV) is a major pathogen of cattle and infects a diverse range of ruminants worldwide [1]
Bovine viral diarrhea virus infection in cattle causes a variety of clinical representations, from an asymptomatic infection to fatal disease [4]
A mild pyrexia and transient leukopenia are apparent for 2–3 days with seroconversion usually before 21 days post-infection
Summary
Bovine viral diarrhea virus (BVDV) is a major pathogen of cattle and infects a diverse range of ruminants worldwide [1]. It is a member of the family Flaviviridae, genus pestivirus that includes border disease virus, classical swine fever virus, and atypical pestiviruses. The majority of BVD control/eradication programs aim to identify these animals through the use of appropriate diagnostic assays and remove them from herds. This in conjunction with vaccination schedules reduces horizontal transmission to naïve herd mates and prevents vertical transmission to the fetus. It is necessary to ensure that BVD vaccines afford protection to circulating strains of BVDV-1 and -2
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