Establishment of a bovine adenovirus type 3 (BAV3)-transformed hamster cell line and characterization of BAV3-tumor associated surface antigens.

Abstract

A clonal cell line of transformant, BHKT73, was isolated from BHK21/13 cells infected with bovine adenovirus type 3 (BAV3). BHKT73 cells showed characteristics typical of transformed cells, including the ability to grow in 1.3 % methylcellulose, high agglutinability with lectins, and unclear cytoplasmic microtubular fibers. Cot analysis showed that BHKT73 cells were contain BAV3 DNA.Tumor-associated surface antigens (TASAs) of BHKT73 cells, and TrD-5 and TrJE-6 cells (A31 cells transformed by BAV3 DNA fragments with respec-tive map units of 3.6-19.7 and 0-11.9) were studied by the protein A binding assay with rabbit anti-BHKT73 IgG, BALB/c mice anti-BHKT73 antiserum and BALB/c mice anti-TrD-5 and TrJE-6 antisera. All these antisera bound to the surfaces of BHKT73, TrD-5 and TrJE-6 cells, but not to the surfaces of SV40-and murine sarcoma virus-transformed A31 cells. They also bound to the surface of polyomavirus-transformed cells (pyBHK and PV-4). After absorp-tion with pyBHK cells, these antisera showed decreased ability to bind to the surfaces of BHKT73, TrD-5, PV-4 and pyBHK cells. These results indicate that all the genetic information of BAV3 that is required for the expression of BAV3-TASAs is included between the map units of 3.6 and 11.9 on the BAV3 gene, and that BAV3-TASAs may have antigenic determi-nant site(s) common to one of the TASAs on polyomavirus-transformed cells.