Abstract
Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D organoid cells. It costs a lot of money and consumes more time and effort compared with 2D cultured cells. Therefore, the establishment of cheaper and Matrigel-free organoid culture that can maintain the characteristics of a part of 3D organoids is demanded. In the previous study, we established a dog bladder cancer (BC) 3D organoid culture system by using their urine samples. Here, we successfully isolated cells named “2.5D organoid” from multiple strains of dog BC 3D organoids using 2.5 organoid media. The cell proliferation speed of 2.5D organoids was faster than parental 3D organoid cells. The expression pattern of stem cell markers was close to 3D organoids. Injection of 2.5D organoid cells into immunodeficient mice formed tumors and showed the histopathological characteristics of urothelial carcinoma similar to the injection of dog BC 3D organoids. The 2.5D organoids had a similar sensitivity profile for anti-cancer drug treatment to their parental 3D organoids. These data suggest that our established 2.5D organoid culture method might become a reasonable and useful tool instead of 3D organoids in dog BC research and therapy.
Highlights
Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine
We established a novel method of 3D organoid culture from bladder cancer (BC) diseased dogs using their urine samples[14]
We confirmed that 2.5D organoids can be isolated from different strains of dog BC 3D organoids (Fig. 1C)
Summary
Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. The 2.5D organoids had a similar sensitivity profile for anti-cancer drug treatment to their parental 3D organoids These data suggest that our established 2.5D organoid culture method might become a reasonable and useful tool instead of 3D organoids in dog BC research and therapy. Three-dimensional (3D) organoid culture is derived from self-renewing stem cells that typically recapitulate the in vivo architecture, functions, and genetic and molecular imprints of their original tissues It holds great promise for use in medical research for establishing novel personalized therapies, especially cancer[12,13]. Using the 3D organoid culture method for experiments costs a lot of money due to the expensive gel and the stem cell-stimulating supplements contained in the culture media It needs a long time because of the complicated handling (dissolving and solidifying gel) and the slow growth speed of the organoid cells compared with 2D cell lines. There is a need to establish a cheaper and easier-to-use organoid culture method, an alternative to the 3D organoid culture method has not been fully developed
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