Abstract
We explored optimized induction and differentiation medium and culture conditions to establish a protocol of rapid clonal propagation of Ligusticum chuanxiong Hort.The petioles were the best explant and Murashige and Skoog(MS) medium containing 2.0 mg/L KT and 0.5 mg/L IAA was suitable for high-frequency shoot regeneration.The results suggested that callus were induced from petioles of aseptic seedlings in dark conditions.And then these callus were subcultured for 15 days in light conditions,about 44.4% frequency of shoot regeneration was induced.The rooting percentage of the regenerated shoots reached 90% on 1/2 MS supplemented with 0.5 mg/L NAA and 0.5 mg/L IBA.Approximately 95% rooted shoots survived in a greenhouse.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
