Establishment and Optimization of Genetic Transformation and Introduction of TaNHX2 Gene by Agrobacterium tumefaciens in Albizia julibrissin

Abstract

Some factors influencing the transformation efficiency,such as the concentration and infection duration of Agrobacterium tumefaciens,pre-culture and co-culture duration,were optimized to establish a stable Agrobacterium mediated gene transformation protocol of Albizia julibrissin Duraxx.The TaNHX2 gene was transformed into A.julibrissin.Mediated by A.tumefaciens,lots of regenerated plants resistant to Kanamycin(Kan) were obtained.PCR analysis and real time PCR detection of some transgenic plants showed that the TaNHX2 gene was evidently integrated into the genome of transgenic plants and could be transcribed properly.