Establishment and observational studies of deep venous thrombosis in rats model

Abstract

Objective To establish deep venous thrombosis (DVT) model in rats, and analyze the changes of prothrombin time, fibrinogen content, D-dimer content and thrombus length before and after operation. Methods All of 30 SD rats were ligatured and blocked the inferior vena cava and its branches, and injured vein wall by micro vessel clamp method to induce the formation of deep venous thrombosis. Venous blood were collected to detected the fibrinogen content, D-dimer content, and prothrombin time at 1 day before operation and postoperative 1, 4, 7, 10, 14, 21 days. The angiography performed to observe the thrombosis and blood vessel recanalization. Results A total of 23 deep venous thrombosis models were successfully produced, with a power of 76.67% (23/30). Prothrombin time, fibrinogen content and D-dimer content in the preoperative and postoperative were statistically significant, but the differences were not statistically significant between different time after operation. There were significant differences in the length of thrombus in different time. Conclusions Prothrombin time, fibrinogen content and D-dimer content can be used as important reference indexes in the formation of thrombosis, but in the evaluation of disease progression and severity of the value were limited. The thrombus length increased with time, then dissolved, and disappeared. Key words: Rats; Model, animal; Fibrinogen; D-dimer