Abstract
Objective: We established a novel procedure to generate a reversible partial unilateral ureteral obstruction (PUUO) in rabbit. The method allows us to reliably measure the degree of ureteral obstruction in live animals, and thus could be a useful tool for studying kidney diseases.Methods: Thirty rabbits of clean grade were divided randomly into sham control group and obstruction (PUUO) group. Each rabbit in this study received the same blocking surgery, in which the upper ureter was curvilinearly incised and inserted with two F6 ureteral catheters that were connected with a tee joint valve. Ureteral obstruction was created and released through the valve adjustment. Serum creatinine and ultrasonographic measurements were performed preoperatively, on the fifth and tenth days after obstruction surgery, and on the 10th and 20th day, respectively, after the relief of the obstruction. Pathological measurements were taken in two randomly chosen rabbits of each group on the 10th day after surgery and on the 20th day after obstruction relief.Results: Data showed that the serum creatinine went transiently up and down in the early days and then remained a little bit higher in the following days after obstruction surgery. The morphology in obstructed kidney changed significantly on the 10th day postoperatively, compared to the sham control group. The obvious differences were also observed in pathology tests. After the relief of obstruction, the volume of renal pelvis (V), renal cortical thickness (RCT), and pathological impairment were partially reversed.Conclusions: Those findings indicate our procedure generate a successful and reversible PUUO animal model. It is a reliable and simple procedure for generating an animal model for reversible PUUO. The feasibility and significance of the new method was confirmed through ultrasonographic and pathological results.
Highlights
The obstructive nephropathy is caused by the lesion of an occlusive urinary track and/or its neighboring structures
There was no significant difference of the pre-operative serum creatinine in the partial unilateral ureteral obstruction (PUUO) (111.15 ± 12.36 lM) and sham control group (114.16 ± 10.38 lM) (p > .05)
Creatinine levels of the PUUO group (128.44 ± 12.28 lM) went upward and reached significance compared to the sham control group (115.41 ± 11.00 lM) on the 5th day postoperatively
Summary
The obstructive nephropathy is caused by the lesion of an occlusive urinary track and/or its neighboring structures. The unilateral ureteral obstruction (UUO) model sits at the interface between acute kidney injury (AKI) and chronic kidney disease (CKD). Persistence of the obstruction leads to histological features of CKD in one to two weeks.[1,2] Clinically, the patients of partial unilateral ureteral obstruction (PUUO) have taken a great part of all the sufferers in unilateral ureteral obstruction. Experimental PUUO is an animal model of human disease, which reproduces ureteral obstruction induced by kidney injury in human, and models several key pathogenic processes of AKI and CKD, including tubular cell injury, interstitial inflammation and fibrosis.[1] For these reasons, the PUUO model is a popular experimental model for obstructive nephropathy. The reversible PUUO models in this manuscript can mimic the entire lesion or recovery process of obstructive nephropathy in clinical settings
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