Abstract
BackgroundBreast cancer is one of the most common cancers among women throughout the world. Therefore, established cell lines are widely used as in vitro experimental models in cancer research.MethodsTwo continuous human breast cell lines, designated MBC1 and MBC2, were successfully established and characterized from invasive ductal breast carcinoma tissues of Malaysian patients. MBC1 and MBC2 have been characterized in terms of morphology analysis, population doubling time, clonogenic formation, wound healing assay, invasion assay, cell cycle, DNA profiling, fluorescence immunocytochemistry, Western blotting and karyotyping.ResultsMBC1 and MBC2 exhibited adherent monolayer epithelial morphology at a passage number of 150. Receptor status of MBC1 and MBC2 show (ER+, PR+, HER2+) and (ER+, PR-, HER2+), respectively. These results are in discordance with histopathological studies of the tumoral tissues, which were triple negative and (ER-, PR-, HER2+) for MBC1 and MBC2, respectively. Both cell lines were capable of growing in soft agar culture, which suggests their metastatic potential. The MBC1 and MBC2 metaphase spreads showed an abnormal karyotype, including hyperdiploidy and complex rearrangements with modes of 52–58 chromosomes per cell.ConclusionsLoss or gain in secondary properties, deregulation and specific genetic changes possibly conferred receptor changes during the culturing of tumoral cells. Thus, we hypothesize that, among heterogenous tumoral cells, only a small minority of ER+/PR+/HER2+ and ER+/PR-/HER2+ cells with lower energy metabolism might survive and adjust easily to in vitro conditions. These cell lines will pave the way for new perspectives in genetic and biological investigations, drug resistance and chemotherapy studies, and would serve as prototype models in Malaysian breast carcinogenesis investigations.
Highlights
IntroductionBreast cancer is the most common cancer among Malaysian women
Breast cancer is the most common cancer among women throughout the world
Histopathology study Histological examination of the breast tissues showed that ER, PR and HER2/neu status of tumoral tissue for MBC1 was ER, PR- and HER2- (Triple negative), while for MBC2 was ER, PR- and HER2+ (Data not shown)
Summary
Breast cancer is the most common cancer among Malaysian women. The peak age for cancer cases among the three major ethnic groups, namely Malay, Chinese and Indian was between 40 to 49 years [2]. Malay women presented larger tumours, in which 50% to 60% were at the later stages (3 and 4), compared to other ethnic groups [3]. The establishment of breast cancer cell lines is an important tool to understand biological processes involved in this disease, as well as the identification of potential therapeutic targets [4]. Breast cancer is one of the most common cancers among women throughout the world. Established cell lines are widely used as in vitro experimental models in cancer research
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