Abstract
Establishment and characterization of paired primary cultures of human pancreatic cancer cells and stellate cells derived from the same tumor
Highlights
Pancreatic ductal adenocarcinoma (PDAC), commonly known as pancreatic cancer, is one of the most lethal solid tumors, characterized by early metastasis, a complex tumor microenvironment, profound chemoresistance, and overall 5-year survival of less than 7% [1,2]
Reagents were purchased from the following sources: Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L glucose, penicillin-streptomycin (Pen-Strep), Amphotericin B, Trypsin/EDTA, fetal bovine serum (FBS), and PierceTM BCA protein assay kit from Thermo Fisher Scientific (Waltham, MA, USA); bovine serum albumin (BSA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), and phosphate-buffered saline (PBS) from Sigma-Aldrich (St Louis, MO, USA); and Ultima Gold from Perkin Elmer (Waltham, MA, USA)
The study of the cellular interactions taking place between the cancer and stroma has recently emerged as an important topic in pancreatic cancer research [43,44,45]
Summary
Pancreatic ductal adenocarcinoma (PDAC), commonly known as pancreatic cancer, is one of the most lethal solid tumors, characterized by early metastasis, a complex tumor microenvironment, profound chemoresistance, and overall 5-year survival of less than 7% [1,2]. Despite new insight in the genomic basis of the disease, therapeutic advancement for PDAC has been negligible over the past decades [3,4,5]. PDAC is the fourth most common cause of cancer deaths in the Western world and is expected to rank second by 2030 [6]. The profound resistance of PDAC to conventional chemotherapy is considered a major impediment to improved survival [10]
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