Establishment and characterization of new cell lines of anaplastic pancreatic cancer, which is a rare malignancy: OCUP-A1 and OCUP-A2.

Kotaro Miura,Masakazu Yashiro,Ryosuke Amano,Kenjiro Kimura,Tsuyoshi Hasegawa,Masaichi Ohira,Sadaaki Yamazoe,Kohei Nishio,Kosei Hirakawa,Akihiro Murata,Bunzo Nakata,Go Ohira

doi:10.1186/s12885-016-2297-y

Abstract

BackgroundAnaplastic pancreatic cancer (APC) cell lines have been scarcely established.MethodsThe morphology, gene expressions, karyotyping and epithelial-mesenchymal transition markers of newly established APC cell lines OCUP-A1 and OCUP-A2 were analyzed. Their abilities of proliferation under normoxia and hypoxia, migration and invasion were compared to 4 commercially available pancreatic ductal adenocarcinoma (PDA) cell lines. Their induction of angiogenesis, stem-like cell population and subcutaneous tumor growth in nude mice were estimated, comparing 2 PDA cell lines examined here.ResultsOCUP-A1 and OCUP-A2 cells continuously grew with spindle and polygonal shapes, respectively. Gene analysis revealed 9 gene mutations including KRAS and TP53. Karyotyping clarified numerical structural abnormalities in both cells. Loss of E-cadherin and expression of vimentin in both cell lines were observed. The doubling time of both cell lines was approximately 20 h. Proliferation, migration and invasion abilities were not notable compared to other PDA cell lines. However stem-like cell population of both cell lines was superior to a part of PDA cell lines. Moreover OCUP-A1 showed stronger hypoxia tolerance and induction of angiogenesis than other PDA cell lines. The tumorigenicity in vivo of OCUP-A2 was stronger than conventional PDA cell lines.ConclusionsThe OCUP-A1 and OCUP-A2 cell lines of rare malignancies might be useful for investigating the biology of pancreatic cancer.

Highlights

Anaplastic pancreatic cancer (APC) cell lines have been scarcely established
Background the number of treatment strategies has been increasing for pancreatic ductal adenocarcinoma (PDA), it still has poor prognosis
The present study intended to clarify the biological behaviors of APC using two novel APC cell lines established in our institute, compared to conventional PDA

Summary

Introduction

Anaplastic pancreatic cancer (APC) cell lines have been scarcely established. Anaplastic pancreatic cancer (APC) is a rare histopathological type of PDA. In. Japan, approximately 0.1 % of all pancreatic cancer was this type [5]. Several studies have found that APC frequently occurs in patients with an average age of 60 years [8, 9]. The incidence of this tumor is three times higher in men than in women [8]. Computed tomography (CT) has shown that the tumor is likely to show low density in the central region and high density in the marginal region [3].

Methods

Results

Conclusion