Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines.

Florian R L Meyer,Ingrid Walter

doi:10.3390/vetsci3020009

Florian R L Meyer, Ingrid Walter

Open Access

https://doi.org/10.3390/vetsci3020009

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Journal: Veterinary sciences	Publication Date: Jun 1, 2016
Citations: 9	License type: CC BY 4.0

Affiliation: University of Veterinary Medicine Vienna

Abstract

Osteosarcomas are the most abundant form of bone malignancies in multiple species. Canine osteosarcomas are considered a valuable model for human osteosarcomas because of their similar features. Feline osteosarcomas, on the other hand, are rarely studied but have interesting characteristics, such as a better survival prognosis than dogs or humans, and less likelihood of metastasis. To enable experimental approaches to study these differences we have established five new canine osteosarcoma cell lines out of three tumors, COS_1186h, COS_1186w, COS_1189, and COS_1220, one osteosarcoma-derived lung metastasis, COS_1033, and two new feline osteosarcoma cell lines, FOS_1077 and FOS_1140. Their osteogenic and neoplastic origin, as well as their potential to produce calcified structures, was determined by the markers osteocalcin, osteonectin, tissue unspecific alkaline phosphatase, p53, cytokeratin, vimentin, and alizarin red. The newly developed cell lines retained most of their markers in vitro but only spontaneously formed spheroids produced by COS_1189 showed calcification in vitro.

Highlights

Tumors are one of the major diseases in both human and companion animals with an annual incidence rate of about 182/100,000 in humans [1] 381/100,000 in dogs and 156/100,000 of cats [2].When comparing numerical data between human and veterinary patients it has to be considered that a number of unrecorded cases are present in animal patients, in particular in the feline species
Due to of the natural immortality of tumor cells, they retain their in vivo features better than normal cells which have to be artificially immortalized to be used for longer periods of time [3,4,5]
Namely COS_1033, COS_1186w, COS_1189, and COS_1220, showed a fibroblast-like morphology when grown as monolayers on plastic cell culture dishes or glass surfaces irrespective from the tumor subtype (Figure 1)

Summary

Introduction

Tumors are one of the major diseases in both human and companion animals with an annual incidence rate of about 182/100,000 in humans [1] 381/100,000 in dogs and 156/100,000 of cats [2]. When comparing numerical data between human and veterinary patients it has to be considered that a number of unrecorded cases are present in animal patients, in particular in the feline species. It is, of major clinical importance to investigate issues such as tumor progression and metastasis behavior, the main reason for tumor related death, and to test tumor cells for drug response. Due to of the natural immortality of tumor cells, they retain their in vivo features better than normal cells which have to be artificially immortalized to be used for longer periods of time [3,4,5]

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