Abstract
The Songliao Black pig was the first lean-meat maternal breed bred in China. To preserve genetic diversity of this breed, we designed a procedure to use ear marginal tissue of one male pig to establish a fibroblast cell line (designated SBPF18), which was subsequently characterized according to the population doubling time, karyotype analysis, isoenzyme assay, DNA fingerprint and sex determination, meanwhile microbial contamination assays were conducted to demonstrate the cell line was contamination-free. This method is suitable for establishing the fibroblast cell line from small amounts of tissue, and could further facilitate genomic studies and genetic modification.Key words: Songliao Black pig, fibroblast cell line, primary explants technique, cryogenic preservation
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