Establishment and Characterization of a Novel Gill Cell Line, LG-1, from Atlantic Lumpfish (Cyclopterus lumpus L.).

Hilde Sindre,Anita Solhaug,Lene C Hermansen,Maria K Dahle,Marit M Amundsen,Inger Böckerman,Johanna Hol Fosse,Mona C Gjessing

doi:10.3390/cells10092442

Abstract

The use of lumpfish (Cyclopterus lumpus) as a cleaner fish to fight sea lice infestation in farmed Atlantic salmon has become increasingly common. Still, tools to increase our knowledge about lumpfish biology are lacking. Here, we successfully established and characterized the first Lumpfish Gill cell line (LG-1). LG-1 are adherent, homogenous and have a flat, stretched-out and almost transparent appearance. Transmission electron microscopy revealed cellular protrusions and desmosome-like structures that, together with their ability to generate a transcellular epithelial/endothelial resistance, suggest an epithelial or endothelial cell type. Furthermore, the cells exert Cytochrome P450 1A activity. LG-1 supported the propagation of several viruses that may lead to severe infectious diseases with high mortalities in fish farming, including viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). Altogether, our data indicate that the LG-1 cell line originates from an epithelial or endothelial cell type and will be a valuable in vitro research tool to study gill cell function as well as host-pathogen interactions in lumpfish.

Highlights

Apart from a fibroblastic cell line from lumpfish fin, characterized in 1977 [22], no cell lines from lumpfish have been reported. We have addressed this gap and report the development and characterization of a novel gill cell line from lumpfish (LG-1)
Todifferent define optimal growthWe conditions for the LG-1 cells, we evaluated cell 2proliferaat different temperatures
The cell line was defined as an epithelial

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Infestation with salmon lice (Lepeophtheirus salmonis) is one of the biggest challenges in farming of Atlantic salmon (Salmo salar L.). Bath treatment with anti-parasitic pharmaceuticals was the predominant treatment strategy. In the course of the past decades, a biological approach using cleaner fish feeding on lice from infested salmon has become more and more common.

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