Abstract
Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. Our de novo CRC cell line, PUMC-CRC1 (Peking Union Medical College Colorectal Cancer 1) was derived from a 47-year-old Chinese female patient diagnosed with moderately to poorly differentiated colon adenocarcinoma. Multiple experiments were used for full characterization. The new cell line was epithelial-like and was passaged for more than 40 times, with a population doubling time of 44 h in vitro, detected by cell counts. The cells exhibited complicated chromosomal abnormalities. The tumor formation rate in SCID mice was 100%. The xenograft tumor was adenocarcinoma with poor to moderate differentiation by Haematoxylin and Eosin staining (H&E) sections. Immunohistochemistry (IHC) analysis and next-generation sequencing (NGS) revealed microsatellite stable (MSS), APC (p.T1493fs) inactivation, KRAS (p.G12V) activation, and SMAD4 (p.V506A) mutation. Quality control of the cell line proved mycoplasma negative and identical STR profile with that of the original tissue, and no interspecific or intraspecific cross contamination was detected. In conclusion, PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.
Highlights
Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide
During the process of the establishment of the Peking Union Medical College (PUMC)-CRC1 cell line, samples were repeatedly collected for cell species identification, STR analysis and Mycoplasma detection, to ensure that there was no contamination with other cells or foreign microorganisms
The results showed that P53 was diffusely positive, and the proliferation index marker Ki67 was 80% in the xenografts, suggesting poor prognosis
Summary
Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. We established a new cell line, designated PUMC-CRC1, from the fresh tumor tissue of a 47-year-old Chinese female patient with Duke’s C1 and moderately to poorly differentiated adenocarcinoma in the cecum, who underwent right hemicolectomy and didn’t receive any chemotherapy or radiotherapy before and after surgery. The cell morphology, growth characteristics, cytogenetic features, cancer-related gene mutations, in vivo tumorigenicity and IHC markers of the xenografts were characterized This newly established cell line should serve as a useful model for in vitro and in vivo studies of the carcinogenic mechanisms and screening for new therapeutic drugs against colorectal cancer
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