Establishment and characterization of a cell line derived from the kidney of tilapia, Oreochromis niloticus

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 罗非鱼肾脏细胞系的建立及其生物学特性 DOI: 作者: 作者单位: 1. 中国水产科学研究院长江水产研究所, 湖北 武汉 430223;2. 中山大学海洋学院, 广东 珠海 519082 作者简介: 赵建青(1991-),硕士,研究实习员,从事水产养殖病害研究.E-mail:964117942@qq.com;贾鹏(1989-),博士研究生,从事海洋经济鱼类体细胞研究及应用.E-mail:825143529@qq.com 通讯作者: 中图分类号: S947 基金项目: 湖北省科技支撑计划项目（2015BBA234）；国家大宗淡水鱼产业技术体系专项资金（CARS-45-16）；中国水产科学研究院基本科研业务费专项（2017HY-ZD1005）. Establishment and characterization of a cell line derived from the kidney of tilapia, Oreochromis niloticus Author: Affiliation: 1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;2. School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:采用组织块移植法，对尼罗罗非鱼（）的肾脏组织细胞进行原代培养，建立了罗非鱼肾脏细胞系，已稳定传代培养50代以上，命名为TiK。罗非鱼肾脏细胞系为纤维样细胞，其最佳培养基为DMEM，最适培养温度为28℃，最适血清浓度为15%。在最适培养条件下，罗非鱼肾脏细胞系的群体倍增时间为45.8 h。细胞经液氮冷冻保存6个月后进行复苏，经台盼蓝染色，约（89.84±3.48）%的细胞具有活性，复苏后细胞生长旺盛。染色体分析显示，第32代罗非鱼肾脏细胞系染色体数目分布在20~66之间，众数为48。使用本实验室分离鉴定的罗非鱼病毒感染罗非鱼肾脏细胞，可产生典型的细胞病变效应，表明罗非鱼肾脏细胞对该病毒敏感。该细胞系的建立为罗非鱼病毒病防控技术研究提供了重要的实验材料。 Abstract:Tilapia is an important aquaculture species world-wide, and China is the largest producer of farmed tilapia. In recent years, alongside the rapid development of tilapia aquaculture in the world, diseases in this fish have become a great concern, including viral diseases caused by beta Nodavirus, herpes simplex virus, and tilapia lake virus, which lead to huge economic losses. So far, research studies on tilapia viral pathogens are dependent on the E-11 cell line derived from grouper and tilapia primary brain cells; thus, a cell line derived from the host of tilapia needs to be urgently established for applications. Therefore, in the present study, by using tissue explant techniques, a novel cell line (designated TiK) derived from the kidney of tilapia (), was established and fully characterized. The TiK cell line are fibroblast-like cells, which have been stably subcultured for more than 50 passages. The optimal growth conditions for the TiK cell line are DMEM medium, 28℃, and 15% FBS. Under the optimal conditions, the doubling time of TiK cells is about 45.8 h. After 6 months of cryopreservation in liquid nitrogen, TiK cells exhibit a viability of about (89.84±3.48)%, as measured by trypan blue staining. Chromosome analysis of TiK cells showed that the number of chromosomes ranged from 20 to 66, and the modal number is 48 at the 32th passage. The TiK cell line was infected with tilapia virus isolated by our laboratory, and showed typical cytopathic effects, demonstrating that the TiK cell line was susceptible to infection by tilapia viruses. The establishment of the TiK cell line will contribute to research on the control of tilapia viral diseases. 参考文献 相似文献 引证文献