Abstract
Infections caused by Bacillus cereus often occur, causing a certain degree of health hazards and economic losses to people around the world. Rapid quantitative detection is of great significance for prevention. This study aims to develop a simple and rapid quantitative detection method for B. cereus, which can be used to monitor the contamination of B. cereus in food samples. A fluorescence real-time quantitative PCR assay was employed to solve this problem. According to the conservative sequence of the gyrB gene in B. cereus, primers were designed online using primer-blast of the NCBI website. The recombinant plasmid standard was constructed by genetic engineering technology. Reaction parameters of real-time PCR were also optimized in this study. After the establishment of this method, 16 rice samples collected from a canteen were tested, one of which was positive and the content of B. cereus was 7.13 × 102 copies/g. The analysis of the melting curve indicated that the system was specific and sensitive, therefore which made it an effective approach for the rapid quantitative detection of B. cereus in food samples. This result is of particular significance for application of predicting the levels of B. cereus in food and other samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Biochemistry and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
