Abstract
To establish a method for the determination of iodine species in human serum by inductively coupled plasma mass spectrometry(ICP-MS). Total iodine was determined by ICP-MS in helium mode after dilution of serum with tetramethylammonium hydroxide solution. The serum was added to methanol solution to precipitate the protein, and the supernatant was separated by liquid chromatography(LC) followed by ICP-MS for the determination of inorganic iodine. The limit of detection(LOD) of serum I~- by ICP-MS was 0.17 μg/L, the limit of quantification(LOQ) was 0.57 μg/L, and the linear correlation coefficient R~2=0.9998; the LOD of serum IO_3~- was 0.16 μg/L, the LOQ was 0.55 μg/L, and the linear correlation coefficient R~2=0.9998.The I~- recoveries were 96.2%-104.5% for the total serum iodine assay and 93.7%-98.6% for the inorganic iodine assay. Analysis of the iodine species of the actual serum samples showed that the I~- content was 2.6-12.2 μg/L, the organic iodine content was 45.3-66.0 μg/L, and the serum samples were essentially free of IO_3~-. A convenient, efficient and accurate method for the determination of serum iodine species was established using high performance liquid chromatography tandem with inductively coupled plasma mass spectrometry.
Published Version
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