Abstract
Objective To establish a fluorescent antibody virus neutralization (FAVN) test for hantavirus (HV). Methods With the method of dilution serum and fixed virus, the serum samples were diluted with different concentration, and with virus-liquid were mixed in 96 culture plate hole, 37 ℃ for 1 hour. Then the mixture of serum and virus was added to Vero-E6 cells which had grown into a monolayer, and cultivated in the 37 ℃, 5% CO2 incubator for 7 days; the 96-well plate was fixed and stained. The results were observed under a fluorescence microscope. The neutralization titer was calculated according to the formula. Results The suitable cell concentrations is 4×104~4.5×104/hole, and the proper temperature for neutralize is 37 ℃. 30 serum specimens of HFRS were tested by FAVN and PRNT, the FAVN is more sensitive and stable. Conclusion FAVN is easier, faster and more sensitive than the others. Key words: Hemorrhagic fever with renal syndromes; Hantavirus; Fluorescent antibody virus neutralization
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