Establishing the combined bacterial detection procedure for the diagnosis of bacterial vaginosis

Abstract

Objective To discuss the best bacterial combination for the diagnosis of Bacterial vaginosis (BV). Methods This is a retrospective study, 230 BV-positive patients and 360 healthy women were enrolled based on the Amsel criteria and Nugent score.5 BV-associated bacteria, including Gardnerella vaginalis, Atopobium vaginae and Leptotrichia/Sneathia species, et al. were amplified by specific-PCR assay, the detection rate were compared between two groups.ROC curve and Kappa test were used to select the best combination. Results The detection rate of Gardnerella vaginalis, Atopobium vaginae, Leptotrichia/Sneathia species, Megasphaera species and Mobiluncus mulieris in BV group (91.3%，83.5%，39.1%，42.6% and 36.5% respectively) were markedly higher than that in healthy women (37.2%，14.4%，11.7%，8.9% and 5.6% respectively), χ2 value were 168.848，275.776，60.949，92.886 and 92.68, all P<0.05.The area under ROC curve of A.vag，G.Vag+A.vag，G.Vag+A.vag+Lepto，G.Vag+A.vag+Mega and G.Vag+A.vag+M.mul were 0.845，0.862，0.865，0.869 and 0.867, and the sensitivity and specifity were higher than 80%, the value of Kappa were larger than 0.75 (P<0.05).Thus, these five methods were coincident. Conclusion Detection of Atopobium vaginae may be a better way for the diagnosis of BV.（Chin J Lab Med,2012,35:1167-1171） Key words: Vaginosis; bacterial; Actinobacteria; Leptotrichia; Polymerase chain reaction