Abstract
Background Aims: To establish and validate the method used to analyze cholinesterase in blood and to establish a baseline level among Jordanians living in a heavy agricultural activity area and as well as among those living in an urban non-agricultural area. Materials and Methods: Modified Ellman procedure was used to analyze 851 and 1033 blood samples from heavy agricultural activity and urban area for cholinesterase activity in erythrocytes and Plasma. Results: sample collected from heavy agricultural activity showed low cholinesterase level average 1037U/L ± 279 and 23 U/gHb ± 7.9 compared with urban area average 1616 U/L ± 662 and 31 U/gHb ± 13 for plasma and erythrocyte respectively. The levels in workers living in heavy agriculture activity showed 52% inhibition in plasma and 41.1% in erythrocyte when compared to the established normal levels that were observed in urban area. Conclusions: Clinicians using cholinesterase for clinical diagnosis and management should be aware that baseline levels are different according to the residence of their patients.
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