Establishing reference intervals of the routine laboratory tests for hepatorenal function in Shanghai by biochemistry

Abstract

Objective To establish reference intervals of nine laboratory projects for hepatorenal function and verify its reliability by indirect method. Methods ALL test results were extracted from physical examinations that were stored in the laboratory information system of Zhongshan Hospital during 2012 to 2014. Using Skewness-Kurtosis test to detect the normality of data, if not the original data were transformed through BOX-COX transformation to obtain an approximatenormal distribution. Outliers were identified and omitted by Turkey method. The indirect reference intervals were taken by applying Hoffmann method. The reference change value (RCV) was selected to inspect the statistical significance between the calculated and published reference intervals. Results Among those nine laboratory projects, thedifferences betweenthereference intervalsfor ALT, AST, AKP, GGT, TP, BUNwerelessthan their RCV( 26.65%, 9.92%) , and there was a significant difference between the calculated reference intervals and the current reference interval in the laboratory for the lower limit of the UA of male(>26.65%). Conclusion This research further proved the reliability of indirect reference intervals and this technique deserved to be promoted and applied by other clinical laboratories.(Chin J Lab Med, 2016, 39: 906-910) Key words: Liver function tests; Kidney function tests; Reference values