Establishing a tree shrew model of systemic lupus erythematosus and cell transplantation treatment.

Guang-Ping Ruan,Jian-Yong Yang,Ju-Fen Liu,Zi-An Li,Xiang Yao,Xing-Hua Pan,Jie He,Rong-Qing Pang

doi:10.1186/s13287-016-0385-1

Guang-Ping Ruan, Jian-Yong Yang + Show 6 more

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https://doi.org/10.1186/s13287-016-0385-1

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Abstract

BackgroundThe establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases.MethodsEighty tree shrews were randomly divided into four groups receiving either an intraperitoneal injection of pristane, lipopolysaccharide (LPS), or pristane and LPS, or no injection. Three weeks after injection, the SLE model tree shrews were divided into the model group and the treatment group. Tree shrews in the treatment group and the normal control group were infused with umbilical cord mesenchymal stem cells (UC-MSCs). The cells were labeled with DiR. Two weeks after transplantation, three groups of tree shrews were analyzed for urine protein, serum antinuclear antibodies and antiphospholipid, and inflammatory cytokine antibody microarray detection. The heart, liver, spleen, lung, and kidney were collected from the three groups and subjected to hematoxylin and eosin (HE) staining and detection of renal immune complex deposition.ResultsHE staining indicated pathology in the model group. Red fluorescence revealed immune complex deposition in the kidneys from the model group.ConclusionsThe combined intraperitoneal injection of pristane and LPS is the best way to induce SLE pathological changes. The pathological changes improved after UC-MSC treatment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-016-0385-1) contains supplementary material, which is available to authorized users.

Highlights

The establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases
The tree shrew model developed in this study aims to elucidate the exact mechanism of lupus nephritis and the mechanisms and efficacy of UCMSCs in the treatment of SLE
These results showed that the UC-Mesenchymal stem cell (MSC) of the tree shrews separated and cultured had MSC surface markers (Fig. 1)

Summary

Introduction

The establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases. Systemic lupus erythematosus (SLE) is an autoimmune disease of unknown etiology. A variety of autoantibodies and immune complexes appear in the serum of SLE patients, along with severe glomerulonephritis [1]. SLE therapy – cyclophosphamide therapy – can control most lupus nephritis well, but not all cases can be controlled. Better understanding of the pathogenesis of the disease is very important to improve the therapeutic effect of SLE. SLE has a complex etiology, and the exact mechanism of this autoimmune disease has not been elucidated. A susceptibility gene regulates the pathogenesis of SLE, and lymphocytes and cytokines participate in abnormal immune responses, which significantly increase autoantibodies and damage to the body

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